Virological analysis of a surveillance of measles in Shanghai, 2012-2014
LI Chongshan1,*, SUN Xiaodong1,*, TANG Wei1, WANG Huiling2, LI Zhi1, WANG Jing1, TANG Suwen1, YANG Yuying1, ZHANG Yan2, ZHANG Xi1, YUAN Zheng'an1, XU Wenbo2
1. Shanghai Municipal Center for Disease Control and Prevention, Shanghai 200336, China; 2. World Health Organization Regional Office for Western Pacific Regional Reference Measles, Laboratory and State Key Laboratory for Molecular Virology & Genetic Engineering, Institute for Viral Disease Control and Prevention, Chinese Centre for Disease Control and Prevention, Beijing 102206, China
Abstract：A 3-year retrospective study was performed based on virological surveillance data of measles virus in Shanghai from 2012 to 2014. Vero/Slam cell line was chosen to isolate virus of throat swabs collected from suspected measles cases. The fragment of Cterminal of nucleoprotein was amplified by reverse transcriptasepolymerase chain reaction (RT-PCR), and the products were directly sequenced. The phylogenetic analysis of gene relationship was performed based on the 450 nucleotides of Cterminal of nucleoprotein. A total of 710 measles virus strains were isolated from 2 562 throat swab specimens (27.71%). Almost all the isolates were subgenotype H1a, except 1 genotype D8 and 8 genotype B3. The identity of subgenotype H1a strains was 96.0%-98.0% for nucleotide sequence and 92.7%-98.0% for amino acid sequence respectively when compared with a World Health Organization (WHO) representative reference strain (GenBank No. AF045212). Whereas the nucleotide sequence identity and amino acid sequence identity between genotype D8 and WHO representative reference strain (GenBank No. AF280803) and between genotype B3 and WHO representative reference strain (GenBank No. L46753) were 97.8%, 98.6% and 96.7%-96.9%, 95.3%, respectively. The results suggest that subgenotype H1a measles virus is predominant in Shanghai from 2012 to 2014. Imported genotype D8 and B3 measles virus strains are first found in Mainland China through Shanghai measles surveillance system.
李崇山1,*，孙晓冬1,*，唐伟1，王慧玲2，李智1，王静1，汤素文1，杨玉颖1，张燕2，张曦1，袁政安1，许文波2. 2012—2014年上海市麻疹病毒学监测情况分析[J]. 微生物与感染, 2016, 11(1): 12-17.
LI Chongshan1,*, SUN Xiaodong1,*, TANG Wei1, WANG Huilling2, LI Zhi1, WANG Jing1, TANG Suwen1, YANG Yuying1, ZHANG Yan2, ZHANG Xi1, YUAN Zheng'an1, XU Wenbo2. Virological analysis of a surveillance of measles in Shanghai, 2012-2014. JOURNAL OF MICROBES AND INFECTIONS, 2016, 11(1): 12-17.
