Immunological evaluation of BALB/c mice as an animal model for herpes simplex virus 1 infection
ZHANG Xiaolong1,*, TANG Beibei1,*, HE Yufeng1, DUAN Yongzhong1, WANG Lichun1, ZHANG Zhixiao1, ZHOU Jumin2, LI Qihan1
1. Yunnan Key Laboratory of Vaccine Research and Development on Severe Infectious Disease, Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, 650118, China; 2. Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Kunming 650223, China
Abstract：To investigate the immunological responses of BALB/c mice to herpes simplex virus type 1 (HSV-1) infection, the following factors were followed: strains, infect routes and mouse ages, the dendritic cells (DCs) and HSV-1 specific T cell rate and function in peripheral blood mononuclear cells (PBMCs), serum neutralizing antibody titer, and CD8＋ T cell infiltration in nervous tissues in HSV-1-infected BALB/c mice. The results showed that 3-week- and 6-week-old BALB/c mice challenged with HSV-1 Mckrae and 17＋ strains through corneal and nasal routes led to an increased rate of DCs in PBMCs, and conferred DC capacity to stimulate HSV-1 specific T cells in vivo. At 35 d post infection, low level interferon γ (IFN-γ)＋ T cells and no interleukin 4 (IL4)＋ T cells could be detected in PBMCs, and low level neutralizing antibody was present or absent in serum. Ninety days after BALB/c mice challenged with HSV-1 through subcutaneous and foot pad injection, CD8＋ T cells could be detected in mouse trigeminal ganglia in different groups. However, HSV-1 specific IFN-γ＋ T cell and IL4＋ T cell rates showed divergence between different virus strains and mouse ages. Foot pad injection of BALB/c mice with HSV-1 could elicit higher neutralizing antibody titer compared with subcutaneous injection. This research demonstrated that, infection of different age BALB/c mice with different HSV-1 strains through different routes can stimulate comparable DC maturation and antigen presentation.
张小龙1,*，汤贝贝1,*，何玉凤1，段永忠1，王丽春1，张志晓1，周巨民2，李琦涵1. BALB/c小鼠作为单纯疱疹病毒1型感染模型的免疫学分析[J]. 微生物与感染, 2017, 12(4): 222-228.
ZHANG Xiaolong1,*, TANG Beibei1,*, HE Yufeng1, DUAN Yongzhong1, WANG Lichun1, ZHANG Zhixiao1, ZHOU Jumin2, LI Qihan1. Immunological evaluation of BALB/c mice as an animal model for herpes simplex virus 1 infection. JOURNAL OF MICROBES AND INFECTIONS, 2017, 12(4): 222-228.