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微生物与感染
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外环境压力对福氏志贺菌荚膜异多糖酸合成调节子转录的影响
叶立娜,蔡霞,林志伟,吕智慧,王小飞,尚永朋,瞿涤
复旦大学基础医学院病原生物学系,医学分子病毒学教育部/卫生部重点实验室,上海 200032
Impact of environmental stresses on transcriptional regulation of Rcs in Shigella flexneri
YE Li-Na, CAI Xia, LIN Zhiwei, LV Zhihui, WANG Xiaofei, SHANG Yongpeng, QU Di
Department of Medical Microbiology and Parasitology,Key Laboratory of Medical Molecular Virology of MOE & MOH,School of Basic Medical Sciences,Fudan University,Shanghai 200032,China
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摘要: 荚膜异多糖酸合成调节子(regulator of colanic acid capsule synthesis,Rcs)对大肠埃希菌适应外环境压力具有重要调控功能,但其在志贺菌中的功能尚未见报道。为探索外环境压力对福氏志贺菌Rcs编码基因rcs转录水平的影响,本研究采用核苷酸序列比对及蛋白结构域预测等生物信息学方法分析福氏志贺菌的Rcs编码基因簇rcsBDC,利用实时定量聚合酶链反应(quantitative real-time polymerase chain reaction,qRT-PCR),对该菌不同生长时期的rcsBrcsDrcsC基因转录水平进行分析,并检测在不同pH值培养基、渗透压条件下的基因转录水平。结果显示,福氏志贺菌rcsBDC在培养5~6 h(对数中期)时转录水平较高,8~10 h(稳定期)时转录水平较低(P<0.001);10 h时,rcsBrcsD在酸性、渗透压条件下的转录水平均显著高于正常条件下的转录水平(P<0.05)。结果提示,外环境刺激可提高福氏志贺菌在稳定期的rcsBrcsD转录水平,为志贺菌适应胃肠道酸性、渗透压环境的机制研究提供了一定理论基础。

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叶立娜
蔡霞
林志伟
吕智慧
王小飞
尚永朋
瞿涤
关键词 荚膜异多糖酸合成调节子福氏志贺菌双组分信号转导系统外环境压力    
Abstract:The regulator of colanic acid capsule synthesis (Rcs) is a complicated two-component signal transduction system. Although details of Rcs in most Enterobacteria are known, its regulation in Shigella has not been reported yet. This study aims to explore the influences of environmental stresses on Rcs transcription in Shigella. Based on biological informatics analysis of RcsBDC in Shigella flexneri 2a 301, the transcriptional levels of rcsB, rcsD, rcsC at different growth stages were analyzed by real-time polymerase chain reaction (PCR), and the changes in the transcriptional levels of the three genes in acid and osmotic pressure conditions were examined. The transcriptional levels of the three genes were higher in 5-6 h (logarithmic phase), and lower in 8-10 h (stationary phase). The transcriptional levels of rcsB and rcsD in acid and osmotic pressure conditions were higher than in normal condition, suggesting environmental stimuli promote the transcriptional levels of rcsB and rcsD in Shigella flexneri during stationary phase.

Key wordsRcs    Shigella flexneri    Two-component signal transduction system    Environmental stress
基金资助:国家自然科学基金(81571955)

通讯作者: 瞿涤   
引用本文:   
叶立娜,蔡霞,林志伟,吕智慧,王小飞,尚永朋,瞿涤. 外环境压力对福氏志贺菌荚膜异多糖酸合成调节子转录的影响[J]. 微生物与感染, 2018, 13(3): 155-164.
YE Li-Na, CAI Xia, LIN Zhiwei, LV Zhihui, WANG Xiaofei, SHANG Yongpeng, QU Di. Impact of environmental stresses on transcriptional regulation of Rcs in Shigella flexneri. JOURNAL OF MICROBES AND INFECTIONS, 2018, 13(3): 155-164.
链接本文:  
http://jmi.fudan.edu.cn/CN/10.3969/j.issn.1673-6184.2018.03.004     或     http://jmi.fudan.edu.cn/CN/Y2018/V13/I3/155

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