Abstract：Batches of Oka varicella vaccine prepared in different years by Shanghai Institute of Biological Products Co., Ltd. were deep-sequenced by the next-generation sequencing (NGS) technology and analyzed to evaluate the quality consistency in molecular level. In average there were 77 loci present in Oka genome with proportion of vaccine-type allele (Pv) no less than 10%, of which 76 appeared in at least two batches. The maximum Pv difference of batch-to-batch was less than 10%. There were 40 loci with average Pv no less than 30%, among which 19 led to amino acid changes and 15 located within ORF62 and adjacent non-coding area. The results demonstrate a good molecular consistency of Oka varicella vaccines produced in different years, and provide research methods and basic data for the quality control of vaccine manufacture and market supervision.
王月红，张赛，吴根鹏，王亮，朱为. 二代测序法深度分析水痘疫苗Oka株病毒基因特征[J]. 微生物与感染, 2018, 13(6): 362-367.
WANG Yuehong, ZHANG Sai, WU Gengpeng, WANG Liang, ZHU Wei. Deep analysis of molecular characteristics of Oka strains of varicella vaccine with next-generation sequencing. JOURNAL OF MICROBES AND INFECTIONS, 2018, 13(6): 362-367.
2018, Vol. 13 
