二代测序法深度分析水痘疫苗Oka株病毒基因特征

王月红,张赛,吴根鹏,王亮,朱为

微生物与感染 ›› 2018, Vol. 13 ›› Issue (6) : 362-367.

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微生物与感染 ›› 2018, Vol. 13 ›› Issue (6) : 362-367. DOI: 10.3969/j.issn.1673-6184.2018.06.006
论著

二代测序法深度分析水痘疫苗Oka株病毒基因特征

  • 王月红,张赛,吴根鹏,王亮,朱为
作者信息 +

Deep analysis of molecular characteristics of Oka strains of varicella vaccine with next-generation sequencing

  • WANG Yuehong, ZHANG Sai, WU Gengpeng, WANG Liang, ZHU Wei
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摘要

采用二代测序(next-generation sequencing,NGS)技术,对上海生物制品研究所有限责任公司(简称SIBP)2015—2017这3个年度生产的Oka株水痘疫苗进行深度测序,从分子水平分析疫苗质量的一致性,并与不同厂家疫苗/毒种进行比较,分析它们之间的遗传特征相关性。结果显示,3个年度生产的水痘疫苗中平均有77个位点的疫苗型位点检出频率(proportion of vaccine-type allele,Pv)≥10%,至少在两批疫苗中出现Pv≥10%的位点数共76个,两两之间Pv最大差值<10%,与平均值的最大差值为<5%。共有40个位点的Pv均值≥30%,其中19个位点导致氨基酸残基变异;15个位于ORF62区域。筛选出35个位点,比较5个公司水痘疫苗/毒种的Oka株病毒基因序列,结果显示它们之间存在相关性,同时存在一定的差异。研究表明,SIBP不同年度生产的水痘疫苗Oka株病毒具有良好的分子水平上的一致性,为疫苗质量管理与市场监督提供了方法和基础数据。

Abstract

Batches of Oka varicella vaccine prepared in different years by Shanghai Institute of Biological Products Co., Ltd. were deep-sequenced by the next-generation sequencing (NGS) technology and analyzed to evaluate the quality consistency in molecular level. In average there were 77 loci present in Oka genome with proportion of vaccine-type allele (Pv) no less than 10%, of which 76 appeared in at least two batches. The maximum Pv difference of batch-to-batch was less than 10%. There were 40 loci with average Pv no less than 30%, among which 19 led to amino acid changes and 15 located within ORF62 and adjacent non-coding area. The results demonstrate a good molecular consistency of Oka varicella vaccines produced in different years, and provide research methods and basic data for the quality control of vaccine manufacture and market supervision.

关键词

水痘-带状疱疹病毒 / 水痘疫苗 / 二代测序 / 序列分析 / Oka株

Key words

Varicella-zoster virus / Varicella vaccine / Next-generation sequencing / Sequence analysis / Oka strain

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王月红,张赛,吴根鹏,王亮,朱为. 二代测序法深度分析水痘疫苗Oka株病毒基因特征[J]. 微生物与感染. 2018, 13(6): 362-367 https://doi.org/10.3969/j.issn.1673-6184.2018.06.006
WANG Yuehong, ZHANG Sai, WU Gengpeng, WANG Liang, ZHU Wei. Deep analysis of molecular characteristics of Oka strains of varicella vaccine with next-generation sequencing[J]. Journal of Microbes and Infections. 2018, 13(6): 362-367 https://doi.org/10.3969/j.issn.1673-6184.2018.06.006

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