Microflora and drug sensitivity of external auditory canal secretions collected from healthy people with wet ear
LI Yiqiang1, ZHANG Yinbo1, ZHU Xinyan1, YAN Chenxi1, WANG Peng1, SUN Guiqin1, YU Hong2
1. College of Medical Technology, Zhejiang Chinese Medical University, Hangzhou 310053, Zhejiang Province, China; 2. College of Basic Medical Sciences, Zhejiang Chinese Medical University, Hangzhou 310053, Zhejiang Province, China
Abstract:To understand the quantity, species, and drug sensitivity of bacteria in the external auditory canal of healthy people with wet ear, the secretions of both right and left ears were collected from 38 healthy wet ear people without ear diseases. A total of 76 samples were subjected to standard clinical culture and isolation procedure on LB plates. The total number of colonies were counted, and representative colonies were subjected to species identification (VETEK-2) and drug sensitivity assays (Kirby-Bauer test). The beta-lactamase of penicillin resistant strains was detected by method of cefnithiphene disk. The results showed that the bacterial number in 88.3% of samples were within 1×102 CFU/sample~1×106 CFU/sample. 20 species of bacteria were detected, 97.1% were cocci and 2.9% were bacilli. The top four bacteria were Staphylococcus cephalus, Staphylococcus auriculae, Staphylococcus hominus and Staphylococcus epidermidis. The sensitivity rate to tobramycin, minocycline, vancomycin and furantoin were more than 90.0%, and resistant rate to cotrimoxazole and penicillin was 50%; 25 of 132 Staphylococcus strains were resistant to penicillin and the positive rate of beta-lactamase was 44.0%.
李一强1,章银波1,朱欣琰1,晏晨曦1,王鹏1,孙桂芹1,俞洪2. 湿型外耳道分泌物的菌群结构及其药物敏感性分析[J]. 微生物与感染, 2020, 15(6): 345-353.
LI Yiqiang1, ZHANG Yinbo1, ZHU Xinyan1, YAN Chenxi1, WANG Peng1, SUN Guiqin1, YU Hong2. Microflora and drug sensitivity of external auditory canal secretions collected from healthy people with wet ear. JOURNAL OF MICROBES AND INFECTIONS, 2020, 15(6): 345-353.