Analysis of drug resistance and homology of Carbapenem-resistant Klebsiella pneumonia isolated from bloodstream infection
YANG Siyu1, XU Shuibao1, CHEN Zhong2, ZHANG Wenhong1, JIN Jialin1, CHEN Shu1
1. Department of Infectious Diseases, HuaShan Hospital, Shanghai 20040, China; 2. Key Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai 200030, China
Abstract:Drug resistance and molecular typing of carbapenem-resistant Klebsiella pneumonia(CRKP)isolated from a bloodstream infection was conducted to provide scientific basis for clinical practice, prevention, and control measures. Non-repetitive Klebsiella pneumoniae strains collected in a Shanghai tertiary hospital from April 2015 to March 2018 were subjected to the study. A total of 51 strains of CRKP were isolated. Antimicrobial susceptibility testing was carried out, the carbapenemase genes were detected by PCR, and the homology was analyzed by Pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). The analysis result showed that the CRKP strains were multi-resistant to antibiotics and blaKPC-2 gene were detected in all strains. Producing blaKPC-2 is a major resistant mechanism to carbapenems for Klebsiella pneumoniae. The results of PFGE typing showed there were dominant clones in this hospital, with ST11 being the main type (84.31%). The results indicated that surveillance of bacterial resistance is an effective control for hospital infection management.
杨思宇1,徐水宝1,陈重2,张文宏1,金嘉琳1,陈澍1. 血流感染碳青霉烯类耐药肺炎克雷伯菌的耐药分析及同源性研究[J]. 微生物与感染, 2020, 15(6): 354-359.
YANG Siyu1, XU Shuibao1, CHEN Zhong2, ZHANG Wenhong1, JIN Jialin1, CHEN Shu1. Analysis of drug resistance and homology of Carbapenem-resistant Klebsiella pneumonia isolated from bloodstream infection. JOURNAL OF MICROBES AND INFECTIONS, 2020, 15(6): 354-359.