Abstract：In order to understand the molecular characteristics of a feline infectious peritonitis virus (FIPV) isolated in Shanghai, genes encoding three non-structural proteins ORF3 (3a, 3b and 3c), ORF7 (7a and 7b) and S2 of three FIPV strains were amplified by RT-PCR, sequenced and analyzed. On ORF3a, ORF3b and ORF3c substitutional mutations leading to amino acid changes were identified. On ORF7a gene, mutations were found at the 47th amino acid. A 3 nt deletion in ORF7b gene was identified in one strain. At the S1/S2 cleavage site of the S gene, there were continuous R-R-S/A-R-R-S sequence modules, which were consistent with the protein sequence characteristics of the FIPV cleavage site. The methionine was replaced by leucine at 1 045th amino acid in all samples. The phylogenetic tree showed that the sequences of FIPV ORF3c, ORF7b and S2 genes were closely related to serotype Ⅰ strains, indicating that the three strains belonged to serotype Ⅰ. The mutations in S genes may be involved in the change of tissue tropism in FCoV.
杨德全1，鞠厚斌1，管踦一2，葛菲菲1，李鑫1，沈海潇1，赵洪进1，王建1，周锦萍1. 上海地区3株猫冠状病毒ORF3、ORF7和S2基因的序列测定及进化分析[J]. 微生物与感染, 2020, 15(6): 370-376.
YANG Dequan1, JU Houbin1, GUAN Qiyi2, GE Feifei1, LI Xin1, SHEN Haixiao1, ZHAO Hongjin1, WANG Jian1, ZHOU Jinping1. Sequencing and phylogenetic analysis of ORF3, ORF7 and S2 gene of three feline coronavirus in Shanghai. JOURNAL OF MICROBES AND INFECTIONS, 2020, 15(6): 370-376.
2020, Vol. 15 
