Soluble prokaryotic expression of varicella-zoster virus glycoprotein E without N-terminal signal peptide and its immunogenicity evaluation
WEI Mingtong1, XIA Bingbing2, HE Zhiyuan2, ZHOU Wei2, LIU Xingdong3, ZHAO Jun2，4, CHEN Jingxian4, WANG Mingli2，4
1. The First Clinical Medical College of Anhui Medical University, Hefei 230032, Anhui Province, China; 2. Wuhu Interfell Research Institute, Wuhu 241000, Anhui Province, China; 3. Second Clinical Medical College of Anhui Medical University, Hefei 230032, Anhui Province, China; 4. Institute of Clinical Virology, Anhui Medical University, Hefei 230000, Anhui Province, China
Abstract：Varicella zoster virus (VZV) glycoprotein E (gE) is the main candidate protein for VZV subunit vaccine. Unfortunately, the expression of gE in prokaryotic expression was mainly found in the inclusion body. In this study, a deletion of N-terminal 30 amino acids of gE was constructed on pET32a, expressed in BL21 (DE3). The soluble gE was purified and used to make polyclonal antibodies in BALB/c mice. The titer and specificity of polyclonal antibodies were determined by enzyme linked immunosorbent assay (ELISA) and indirect immunofluorescence. The results showed that the soluble recombinant gE produced by BL21/pET32a-VZV gE system is a practical way for vaccine development.
魏明童1，夏兵兵2，何志远2，周炜2，刘兴东3，赵俊2，4，陈敬贤4，王明丽2，4. 去N端信号肽的水痘-带状疱疹病毒糖蛋白E的原核可溶性表达及其免疫原性评价[J]. 微生物与感染, 2020, 15(6): 377-384.
WEI Mingtong1, XIA Bingbing2, HE Zhiyuan2, ZHOU Wei2, LIU Xingdong3, ZHAO Jun2，4, CHEN Jingxian4, WANG Mingli2，4. Soluble prokaryotic expression of varicella-zoster virus glycoprotein E without N-terminal signal peptide and its immunogenicity evaluation. JOURNAL OF MICROBES AND INFECTIONS, 2020, 15(6): 377-384.
2020, Vol. 15 
