Research on drug resistance characteristics of Corynebacterium striatum
WEI Kongjiao1, WANG Jiazheng2, QIU Xiaotong3, XU Shuai3, ZHU Xiong4, LI Huan4, WANG Xiaoxia4, WANG Chengling1, FAN Shihong1, HAN Lichao3, LIU Zhiguo3, LI Zhenjun1，3
1. Department of Medicine, Tibet University, Lasa 850000, Tibet, China; 2. Department of Clinical Laboratory Medicine, The First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital, Shandong Medicine and Health Key Laboratory of Laboratory Medicine, Jinan 250000, Shandong Province, China; 3. National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China; 4. Central and Clinical Laboratory of Sanya People’s Hospital, Sanya 572000, Hainan Province, China
Abstract：To reveal the drug resistance and its mechanism preliminarily of clinical isolates of Corynebacterium striatum in Shandong Province of China, 83 isolates of Corynebacterium striatum were collected from clinical specimens during March 2017 and June 2020 in a tertiary hospitals from Shandong. The minimal broth dilution method was used to detect the minimal inhibitory concentration.The aminoglycoside modifying enzyme genes [aph(3”)-Ⅰb、aph(6)-Ⅰd、aac(6’)-Ⅰb], the ribosomal protective protein gene of tetracycline resistance (tetW), the ribosomal methylase gene (ermX), quinolone resistance genes (gyrA) were tested by polymerase chain reaction(PCR). The PCR products were sequenced and compared with BLAST.Quantitative real-time PCR (qPCR) was used to analyze the drug-resistant genes ermX and tetW quantitatively, and formula 2－ΔΔCt was used to calculate the relative expression of genes.All the tested Corynebacterium striatum were proved as multi-drug resistant strains. The MDR-Cs showed completely sensitive to vancomycin and linezolid and completely resistance to erythromycin, ciprofloxacin and clindamycin, the detection rate of tetracycline, gentamicin were 86.7% (72/83)and 38.6%(32/83), respectively. The detection rate of ermX and tetW were 100%(83/83) and 88%(73/83), the detection rate of aph(3”)-Ⅰb, aph(6)-Ⅰd, aac(6’)-Ⅰb were 36.1%(30/83), 37.3%(31/83) and 15.7%(13/83), respectively. The resistance phenotype of the strain to erythromycin is cMLS. The drug resistance spectrum is relatively broad in this study, which needs to be paid attention from hospitals and laboratories.
魏孔娇1，王嘉正2，邱小彤3，徐帅3，朱雄4，李欢4，王晓霞4，王成玲1，范仕弘1，韩李超3，刘志国3, 李振军1，3. 纹带棒状杆菌耐药特征研究[J]. 微生物与感染, 2021, 16(6): 384-390.
WEI Kongjiao1, WANG Jiazheng2, QIU Xiaotong3, XU Shuai3, ZHU Xiong4, LI Huan4, WANG Xiaoxia4, WANG Chengling1, FAN Shihong1, HAN Lichao3, LIU Zhiguo3, LI Zhenjun1，3. Research on drug resistance characteristics of Corynebacterium striatum. JOURNAL OF MICROBES AND INFECTIONS, 2021, 16(6): 384-390.
2021, Vol. 16 
