Abstract：The aim of this study was to investigate the effects of Fusobacterium nucleatum (F. nucleatum) on proliferation and apoptosis of human colorectal cancer (CRC) cells as well as its mechanism. Human CRC cells HCT116 at logarithmic growth stage were divided into control group, F. nucleatum group (infected with F. nucleatum), si-NEAT1 group (transfected with si-NEAT1) and F. nucleatum+si-NEAT1 group (infected with F. nucleatum after transfection with si-NEAT1). qRT-PCR was used to measure the level of RNA in cells. CCK-8 assay was performed to detect the proliferation of cells, while flow cytometry was used to calculate the apoptosis rate of cells. The expression levels of apoptosis related proteins caspase-3, Bcl-2 and Bax were determined by western blot. The proliferation of cells was enhanced, and the apoptosis rate of cells was downregulated. The expressions of caspase-3 and Bax were decreased, while the level of Bcl-2 was lifted. In addition, the expression of NEAT1 in CRC cells increased after infection with F. nucleatum. After transfection with si-NEAT1, the proliferation ability of CRC cells was decreased, the apoptosis rate was increased, and the expressions of caspase-3 and Bax protein were added, while the expression of Bcl-2 was reduced. Knockdown NEAT1 expression reversed the effect of F. nucleatum on proliferation and apoptosis of CRC cells. These results suggested that F. nucleatum could promote the proliferation and inhibit the apoptosis of CRC cells by up-regulating of NEAT1.
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