Abstract：Abstract: Objective: To investigate the correlation between exoU gene expression in Pseudomonas aeruginosa and lasI gene deletion and drug resistance. Methods: 640 strains of Pseudomonas aeruginosa (PA) were collected and isolated in our hospital from January 2020 to December 2020, and PCR was used to detect the distribution of exoU virulence genes from different sources of strains, and fluorescence quantitative PCR technology was used to observe the expression of transcriptional regulation related gene exoU and the expression of lasI gene and downstream virulence genes in the strain; The agar dilution method was used to detect the sensitivity of antimicrobial drugs; And Pearson correlation was used to analyze the relationship between the relative expression of each gene. Results: The distribution of 640 PA specimens was mainly sputum (51.56%), blood (22.19%) and pus (14.69%). The exoS+/exoU- gene combination has the highest detection rate in PA samples from all sources, and its detection rates in sputum, blood and pus are 56.97% (188/330), 71.83% (102/142) and 67.01, respectively %(65/97); drug sensitivity results show that 640 strains of PA are resistant to 8 kinds of antibacterial drugs to varying degrees, and the sputum-derived PA strain is more resistant to imipenem than blood, pus and other sources Strain (P<0.001). PA carrying exoU gene has a higher resistance rate to imipenem, aminoglycosides, fluoroquinolones, and macrolide antibacterials (P<0.001); PA carrying exoS gene has a higher resistance to aztreonam Resistance (P<0.05); The resistance of PA with lasI gene deletion to various antibacterial drugs was significantly reduced (P<0.001), and there was a significant negative correlation between the relative expression of the transcriptional regulation-related gene ptrA of the PA strain carrying the exoU gene and exsA and the effector gene exoU (r=-0.645, P<0.001; r=-0.587, P<0.001); There was also a significant negative correlation between the relative expression of the transcriptional regulation-related gene ptrA of the PA strain carrying exoS gene and exsA and the effector gene exoS (r=-0.618, P<0.001; r=-0.532, P<0.001). After lasI gene deletion, the expression levels of downstream virulence genes lasA and aprX decreased significantly (P <0.001), and there was a significant positive correlation between the expression levels of lasA and aprX and the relative expression of lasI gene (r=0.489, P < 0.001; r=0.512, P <0.001). Conclusion: There is a significant correlation between the expression of exoU gene and the drug resistance of Pseudomonas aeruginosa. The expression of virulence genes such as exoU and drug resistance may be related to the negative regulatory gene ptrA; The deletion of the lasI gene will significantly inhibit the expression of virulence genes in the QS system of Pseudomonas aeruginosa and the drug resistance of the bacteria.
张洁莉. 铜绿假单胞菌中exoU基因表达与?lasI基因缺失及耐药的相关性研究[J]. 微生物与感染, 2022, 17(5): 290-296.
zhang jieli. Study on the correlation between exoU gene expression in Pseudomonas aeruginosa and lasI gene deletion and drug resistance. JOURNAL OF MICROBES AND INFECTIONS, 2022, 17(5): 290-296.
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