Abstract:Abstract Objective To analyze the epidemiological characteristics, molecular typing and virulence genes of Salmonella in Ningde area. Methods 364 Salmonella strains were isolated in Ningde area from 2015 to 2020. The epidemiological data was given statistical analysis, and we isolate and identify the strains after culture, use pulse field gel electrophoresis (PFGE) for molecular typing analysis, and use routine PCR to detect virulence genes. Results The results of epidemiological analysis showed that the infection rate of Salmonella in men was significantly higher than that in women (P<0.05), and the infection rate in the 0~10 age group was significantly higher than that in other age groups (P<0.05). A total of 22 serotypes were isolated. Among them, Salmonella typhimurium,Salmonella enteritidis,Salmonella Stanley were the top three serotypes, accounting for 57.42%,16.21%,6.87%, respectively, which were the main serotypes.The results of PFGE typing showed that 364 strains of Salmonella had 212 bands, the similarity of bands was 44.52%~100.00%, and the dominant bands were B53 with 23 strains.The results of virulence gene test showed that the carrying rate of avrA、ssaQ、sodC1、mgtC、gipA was 100.00%, and that of ssiD、spoE、spoB was 90.66%,94.23% and 92.03%, and that of spvC was 63.74%, respectively. Conclusion Salmonella infection in Ningde area is mostly concentrated in children under 10 years of age, and men have high incidence, have certain genetic diversity, and the carrying rate of virulence genes is high. Attention should be paid to this.
Ezat H.Mezal, Rossina Stefanova, Ashraf A. Khan. Isolation and molecular characterization of Salmonella enterica serovar Javiana from food, environmental and clinical samples[J]. International Journal of Food Microbiology, 2013, 164(1).113-118.[J].International Journal of Food Microbiology, 2013, 164(1):113-118