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| Activation analysis of CD4+ and CD8+T cells in peripheral blood and pleural effusion in patients with tuberculous pleurisy |
| ZHU Jie-Hua1， LUO Jun-Min1，YE Jun2，QIN Huan1 , JIANG Juan3, SHAO Yun-Hao4 |
| 1. Department of Immunology, Immunology Innovation Base of Postgraduate Education of Guizhou Province, Zunyi Medical College, Zunyi 563003, China；2. Clinical Laboratory, The Second Affiliated Hospital of Guiyang Medical College, Kaili 550003, China；3. Department of Medical Laboratory, Zunyi Medical College, Zunyi 563003, China；4. Department of Medical Cosmetology, Zunyi Medical College, Zunyi 563003, China |
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Abstract The pathogenesis of tuberculous pleurisy remains unclear. Evidences have shown that immuno-imbalance is an important risk factor for this condition. The present paper aimed to profile CD4 + and CD8 + T cells in patients with tuberculous pleurisy. A total of 30 cases were selected and their peripheral blood and pleural fluid were collected. The mononuclear cells were isolated. The CD4 + cells, CD8 + T cells, activation surface markers of human leukocyte antigen (HLA)-DR, and granzyme B level in CD8+ T cells were detected by flow cytometry. The results showed that the proportions of CD3 + , CD4 + and CD8 + T cells in peripheral blood samples collected from the patients with tuberculous pleurisy were lower than those in the control group. The proportions of CD3 + and CD4 + T cells in patients' pleural effusion were higher than those in peripheral blood, but the proportion of CD8 + T cells had no significant difference. The levels of HLA-DR on CD4 + and CD8 + T cells in peripheral blood in patients with tuberculous pleurisy were higher than those in the control group. And the level of CD8+ granzyme B+ T cells in patients with tuberculous pleurisy was lower than that in the control group. In patients , the levels of CD4 + HLA + , CD8 + HLA + and CD8+ granzyme B+ T cells in pleural effusion were lower than those in peripheral blood. In patients' peripheral blood there was a positive correlation between CD8 + T cells and granzyme B level. The results suggested that the immune response between peripheral blood and pleural fluid in patients with tuberculous pleurisy were different. Immuno-imbalance may play a critical role in the development/prognosis of the disease.
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Received: 28 December 2012
Published: 25 June 2013
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Corresponding Authors:
LUO Jun-Min
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