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::: Office ::: Online Submission Manuscript Tracking Peer Review Editor Work Office Work Editor-in-chief   ::: Journal ::: Forthcoming Articles Current Issue Next Issue Archive Email Alert   Read Articles Download Articles   Quick Search       Advanced Search         2023 Vol.18 No.1 Published 2023-02-25 New Year Greetings Original Article Case Analysis Review ) [HTML 1KB] [PDF 528KB] ( 219 )   Original Article 2 SHI Huichun1, JIAO Fanke2, 3, XU Wei2, 3, CHEN Yongkang2, 3, LU Lu2, 3, ZOU Peng1 Expression and functional study of chimeric antibodies targeting SARS-CoV-2 receptor binding domain Coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection has a serious impact on people’s health and lives. At present, SARS-CoV-2 is still widely spread, but effective treatments for SARS-CoV-2 are still limited. Therefore, it is of great significance to find broad-spectrum neutralizing antibodies to block SARS-CoV-2 infection. In this study, we successfully expressed and purified three human-mouse chimeric antibodies targeting receptor-binding domain (RBD) of SARS-CoV-2, and then the neutralizing activities of these antibodies against a variety of SARS-CoV-2 pseudoviruses and authentic virus were tested. The results showed that these three antibodies could specifically neutralize SARS-CoV-2 wild-type pseudovirus, and the IC50 values were 0.03 μg/mL, 0.06 μg/mL and 0.03 μg/mL, respectively. Furthermore, these antibodies exhibited broad-spectrum neutralizing activity against Alpha, Delta and Lambda pseudoviruses, and could also inhibit the replication of SARS-CoV-2 Delta authentic virus. Mechanism studies showed that these antibodies could block cell-cell fusion mediated by SARS-CoV-2 S protein, and K417, E484 and N501 on receptor-binding domain (RBD) may be the key sites for binding with these three antibodies. 2023 Vol. 18 (1): 2-12 [Abstract] ( 26 ) [HTML 1KB] [PDF 3213KB] ( 317 ) 13 TANG Wanda1, TANG Hailin1, REN Ruiwen2, ZHAO Ping1, ZHAO Lanjuan1 Establishment of a BALB/c mice model of tick-borne encephalitis virus infection An animal model of tick-borne encephalitis virus (TBEV) infection was established to provide a suitable tool for anti-TBEV drug screening. BALB/c mice were inoculated with TBEV by subcutaneous injection, and their symptoms, body weight and survival rate were observed. TBEV antigen distribution, virus titer, pathological changes and the expression levels of tumor necrosis factor α (TNF-α) and interferon β (IFN-β) mRNA were investigated in brain, spleen and kidney of the mice. The results showed that the infected mice had symptoms of arched back and hind limb paralysis. The body weight and survival rate of infected mice were significantly decreased as compared with the uninfected mice. TBEV antigen could be detected in the brain, spleen and kidney of infected mice. Virus titers in the brain were higher than those in the spleen and kidney of infected mice, along with the pathological changes in the brain. The expressions of TNF-α and IFN-β mRNA in the brain, spleen and kidney of mice were associated with the duration of TBEV infection. BALB/c mice model of TBEV infection has been established by subcutaneous injection of virus. 2023 Vol. 18 (1): 13-20 [Abstract] ( 27 ) [HTML 1KB] [PDF 4601KB] ( 117 ) 21 SHI Shujuan, LIU Xiuwen, WENG Yiwei, DANG Juanjuan, LI Zeyu, ZHANG Ying, HUANG Xinxiang Preliminary study on the effect of Salmonella typhi type Ⅵ secretion system on the expression of SopE In order to explore the effect of Salmonella typhi type 6 secretion system (T6SS) on SopE expression and clarify the molecular mechanism, the wild Salmonella typhi strain (WT-pBAD33), T6SS functional molecule-deficient strain (ΔsciG-pBAD33) and complementing strain (C-ΔsciG) carrying sopE∷3×Flag were constructed by suicide plasmid homologous recombination method. The strains were constructed to infect macrophage THP-1 and cultured in simulated macrophage environment (LPM medium, microaerobic). The effect of T6SS on the expression of SopE was investigated by real-time fluorescence quantitative polymerase chain reaction (qRT-PCR) and Western blot experiments. The β-galactosidase activity test further verified the transcription level of sopE gene promoter, and the T6SS functional protein SciG was purified by Ni column. The regulation effect of SciG protein on the sopE gene promoter was analyzed by electrophoretic mobility shift assay (EMSA). The results demonstrated that the expression level of SopE in ΔsciG-pBAD33 was significantly lower than that in WT-pBAD33; SciG protein could not directly bind to the sopE gene promoter region. It was concluded that Salmonella typhi T6SS affects the expression of SopE, but its functional protein SciG can not directly regulate the expression of sopE gene. 2023 Vol. 18 (1): 21-28 [Abstract] ( 33 ) [HTML 1KB] [PDF 2010KB] ( 157 )   Case Analysis 29 LIU Ling, TAN Wenmin, LI Jingcheng, HAN Feng, SANG Shenggang A Salmonella enteritidis strain isolated from the wound secretion of a patient with tibial plateau fracture The clinical microbiology laboratory of the First Affiliated Hospital of Hainan Medical University isolated and cultured a Gram-negative bacillus strain from the wound secretion of a patient with left tibial plateau fracture on June 16, 2022, which was confirmed to be Salmonella entrica subsp. enterica serotype enteritidis by automatic microbial identification system and matrix-assisted laser desorption / ionization time of flight mass spectrometry(MALDI TOF-MS)experiments. The patient was treated with meropenem 1.0 g q8h intravenous drip for 9 d. According to the results of etiological detection and drug susceptible test, the following medication regimen was adopted: piperacillin tazobactam sodium 4.5 g q6h intravenous drip for 5 d, meropenem 1.0 g q8h intravenous drip for 19 d, vancomycin 1.0 g q12h intravenous drip for 10 d, and debridement and drainage of left tibial plateau infection, the patient was cured and discharged. 2023 Vol. 18 (1): 29-34 [Abstract] ( 30 ) [HTML 1KB] [PDF 2209KB] ( 136 )   Review 35 ZHANG Shenyan*，SHEN Zhongliang*，ZHANG Jiming Molecular basis and clinical application of hepatitis B core-related antigen As a recently discovered serological marker, hepatitis B core-related antigen (HBcrAg) consists of hepatitis B core antigen (HBcAg), hepatitis B e antigen (HBeAg) and p22cr. Serum HBcrAg can reflect the level and transcriptional activity of intrahepatic covalently closed circular DNA, as well as predict the natural history of patients with chronic hepatitis B or the efficacy and prognosis of nucleos(t)ide analogues and interferons, including the incidence of clinical outcomes such as relapse and functional cure, thus providing guidance for the determination of the starting point and endpoint of clinical antiviral therapy and the selection of treatment regimens. HBcrAg levels are also associated with the incidence of HBV-associated hepatocellular carcinoma(HCC), which can identify potential high-risk patients for HCC. Therefore, HBcrAg has important clinical application value in the diagnosis and treatment of chronic hepatitis B. 2023 Vol. 18 (1): 35-45 [Abstract] ( 24 ) [HTML 1KB] [PDF 1100KB] ( 237 ) 46 CHEN Shenglin1*, XIAO Man2*, LI Zhaozhong3, XU Zhikai1, HAN Qi1, FENG Dongfang1, DENG Yan1, YIN Zuoming1 Recent progress on cell receptors for SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)is the causative agent of the coronavirus disease 2019(COVID-19), which spreads rapidly in humans and endangers global health and economy. There are no specific and effective antiviral drugs for the treatment of COVID-19 currently. To infect host cells successfully for a virus, receptor binding and virus internalization are considered to be the initial steps. A key to combat SARS-CoV-2 infection is to investigate the viral receptors, which determine its infectivity and host range. The discovery and identification of cell receptors for SARS-CoV-2 will provide new therapeutic targets for COVID-19. This article reviews the recent findings on the entry and attachment receptors for SARS-CoV-2. 2023 Vol. 18 (1): 46-54 [Abstract] ( 19 ) [HTML 1KB] [PDF 772KB] ( 172 ) 55 HE Juanjuan, WANG Xiaolian Progress on bioactivity of lipids of Mycobacterium tuberculosis cell wall Mycobacterium tuberculosis (M. tuberculosis) is the pathogen of tuberculosis, a global epidemic and chronic infectious disease. Its pathogenic mechanism and drug resistance are closely related to its specific cell wall components, especially lipids, such as lipoarabinomannans, trehalose-6, 6’-dimycolates, phenolic glycolipids and other glycolipids. It is of great value to study the lipids of M. tuberculosis cell wall for the diagnosis and prevention of tuberculosis. In order to provide a reference for the related basic research of M. tuberculosis, this review summarized the recent progress on the bioactivity of various lipids of M. tuberculosis cell wall. 2023 Vol. 18 (1): 55-64 [Abstract] ( 21 ) [HTML 1KB] [PDF 2655KB] ( 310 ) Reader Login Author Center Online Submission Author Instruction Layout Art Copyright Agreement News More >>   Other Journal

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