猪流行性感冒病毒H3N2亚型体外诱导猪肺巨噬细胞发生凋亡

祁贤; 姚火春; 陆承平

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微生物与感染 ›› 2009, Vol. 4 ›› Issue (2) : 76-80.

论著

猪流行性感冒病毒H3N2亚型体外诱导猪肺巨噬细胞发生凋亡

祁贤；姚火春；陆承平

作者信息 +

Swine influenza type A H3N2 virus-induced apoptosis in porcine pulmonary alveolar macrophages in vitro

QI Xian；YAO Huo-Chun；LU Cheng-Ping

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文章历史 +

摘要

本文旨在探讨H3N2亚型猪流行性感冒病毒(SIV)引起机体免疫抑制的机制。用SIV分离株体外感染猪肺巨噬细胞(PAM),发现SIV可在PAM中进行生产性复制。感染后96h,病毒的血凝效价达到64。琼脂糖凝胶电泳显示,感染细胞的DNA呈“梯样”图谱;流式细胞仪检测显示,细胞在感染后24h出现明显的亚二倍体DNA峰,72h凋亡细胞达25%。光学显微镜和电子显微镜下可见感染细胞的形态发生特征性改变,如细胞皱缩、细胞表面微绒毛消失、胞质内出现大量空泡、细胞核染色体发生浓缩等。结果提示,SIV可在体外诱导PAM凋亡,推测PAM凋亡可能是SIV引起机体免疫抑制的重要因素之一。

Abstract

Porcine pulmonary alveolar macrophages (PAMs) were infected with A/Swine/Guangdong/1/2004, a swine influenza virus (SIV) isolate. Results showed that A/Swine/Guangdong/1/2004 was able to carry out productive replication in PAMs. DNA laddering in SIV-infected cells showed subdiploid DNA peaks at 24, 48, and 72 h post-infection by flow cytometry. Under light and electron microscopes, morphological changes in SIV-infected cells were observed, including cell shrinkage, plasma membrane blebbing, nuclear condensation, and cytoplasm vacuolization. These results indicate that SIV may induce apoptosis in PAMs in vitro, and apoptosis may play an important role in death of PAMs infected with SIV.