为探讨柯萨奇病毒B3（CVB3）蛋白酶2A是否通过干扰核因子κB（NF-κB）的核定位而影响细胞因子表达，构建CVB3蛋白酶2A的表达载体pcDNA3.1-2A。将此表达载体与核转录因子NF-κB基因启动子荧光素酶报告基因载体pGL3-NF-κB promotor-Luc共转染细胞，经肿瘤坏死因子α（TNF-α）刺激，检测荧光素酶蛋白表达；通过免疫荧光和蛋白免疫印迹检测CVB3蛋白酶2A对NF-κB核定位和二聚体形成的影响。结果显示，CVB3蛋白酶 2A可减少NF-κB二聚体形成，并干扰其核转移。本研究证实，CVB3蛋白酶2A可通过干扰转录因子二聚体形成和核转录而影响细胞因子分泌。

To study the impact of coxsackievirus B3 2A protease on nuclear factor κB (NF-κB ), BHK cells were transfected with a plasmid pcDNA3.1-2A expressing CVB3 2A protease along with pGL3-NF-κB -promotor-luc. The activity of luciferase was detected to identify the relative effect of NF-κB upon tumor necrosis factor α (TNF-α) stimulation. The dimerization of NF-κB /p65 were also detected with Western blotting and translation of NF-κB /p65 was observed by immunofluorescence. The results showed that CVB3 2A protease not only inhibited the expression of luciferase activities from NF-κB /p65 promoter but also interfered the nuclear translocation of NF-κB /p65. CVB3 2A protease still inhibited the dimerization of NF-κB /p65. It is concluded that CVB3 protease 2A inhibits the activation of NF-κB pathway.