目的 分析苏州地区女性人乳头瘤病毒（Human papillomavirus，HPV）各亚型感染分布情况，以及HPV阳性者的宫颈细胞学结果分布，以初步评估感染与细胞学异常的关联，为苏州地区HPV感染的防治提供理论基础。方法 采用荧光定量聚合酶链反应（Fluorescence quantitative polymerase chain reaction，qPCR）对南京中医药大学附属苏州市中医医院及其医联体单位苏州市高新区通安社区卫生院2020—2021年的2852例女性宫颈脱落细胞标本进行检测，分析HPV总感染情况、年龄段感染特点及基因型分布，采用液基薄层细胞学（Liquid-based cytology，TCT）对所有HPV阳性者进行宫颈细胞学检查，并按TBS 2014标准分类。结果 在2852例受检女性患者中，HPV阳性检出502例，感染率为17.60%（502/2852），其中，单一感染447例（89.04%）；多重感染55例（10.96%）。34～39岁女性的HPV感染率为5.75%（164例），40～49岁为3.19%（91例），50～59岁为6.38%（182例），60～65岁为2.28%（65例）。在502例样本中，HPV亚型检出率位居前三位的分别为：HPV52（22.51%，113/502）、HPV58（13.35%，67/502）、HPV16（9.76%，49/502）。502例HPV阳性者中，TCT异常92例（18.33%），其中未明确意义的不典型鳞状上皮细胞（Atypical squamous cells of undetermined significance，ASC-US）71例，低度鳞状上皮内病变（Low-grade squamous intraepithelial lesion，LSIL）19例，高度鳞状上皮内病变（High-grade squamous intraepithelial lesion，HSIL）不典型鳞状上皮细胞-不除外高度鳞状上皮内病变（Atypical squamous cells cannot exclude HSIL，ASC-H）各1例（各占0.20%）。结论 苏州地区的女性HPV阳性率与年龄段存在差异，感染类型主要为高危亚型和单一亚型，优势感染亚型为HPV52、HPV58和HPV16。

Objective To analyze the infection subtype distribution of Human papillomavirus (HPV) among women in Suzhou area, and to describe the cervical cytology findings in HPV-positive women, providing a theoretical basis for the prevention and treatment of HPV infection in Suzhou. Methods Fluorescence quantitative polymerase chain reaction (qPCR) was used to detect 2852 cervical exfoliated cell specimens from women at Affiliated Suzhou Hospital of Traditional Chinese Medicine, Nanjing University of Chinese Medicine, and its medical community unit, Tong'an Community Health Center, Suzhou High-tech Zone. from 2020 to 2021. The total HPV infection, age-specific infection characteristics, and genotype distribution were analyzed. Liquid-based cytology (TCT) was performed for all HPV-positive cases and classified according to TBS 2014. Results Among 2852 female subjects, 502 were HPV positive, with an infection rate of 17.60% (502/2852). Of these, 447 cases (89.04%) were single infections, and 55 cases (10.96%) were multiple infections. The age-specific infection rates were: 164 cases (5.75%) in 34-39 years old, 91 cases (3.19%) in 40-49 years old, 182 cases (6.38%) in 50-59 years old, and 65 cases (2.28%) in 60-65 years old. The top three HPV subtypes detected were: HPV52 (22.51%, 113/502), HPV58 (13.35%, 67/502), and HPV16 (9.76%, 49/502). Among 502 HPV-positive women, there were 71 cases of Atypical squamous cells of undetermined significance (ASC-US), 19 cases of Low-grade squamous intraepithelial lesion (LSIL), 1 case of High-grade squamous intraepithelial lesion (HSIL), and 1 case of Atypical squamous cells cannot exclude HSIL (ASC-H). Conclusion The HPV positive rate among women in Suzhou area varies with age groups. The main infection types are high-risk subtypes and single infections, with HPV52, HPV58, and HPV16 being the predominant subtypes.