本研究旨在设计一款用于呼吸机管路内部消毒的新型装置，并评估其消毒效果。基于射流技术产生过氧化氢干雾的原理，设计了一款串联式干雾过氧化氢呼吸机内管路消毒装置，并选取40台被符合疑似传染病病人诊断标准的患者进行机械通气治疗使用且单机连续使用时间≥72 h的呼吸机作为研究对象，在启动串联式干雾过氧化氢消毒之前、启动串联式干雾过氧化氢消毒1 h、消毒4 h 3个时间节点，分别对呼吸机进气口、出气口、内管道进行取样，并进行菌落总数检测，将细菌总数≤20 CFU /件且不得检出致病菌作为消毒合格的标准，并进行统计学分析。检测结果显示，启动串联式干雾过氧化氢消毒之前合格率为0%，启动串联式干雾过氧化氢消毒1 h和4 h后，各采集部位的菌落数均显著低于消毒前(p＜0.05)，且未检出致病菌，消毒合格率可达到100%。本研究证实串联式干雾过氧化氢呼吸机内管路消毒装置消毒可靠、有效，1 h消毒时长即可达到预期效果。

This study aimed to develop a new disinfection device for the internal tubing of ventilators and evaluate its efficacy. The tandem device was designed to disinfect the internal tubing of ventilators with hydrogen peroxide (H₂O₂) dry mist that was produced by jet technique. Forty ventilators utilized for mechanical ventilation in confirmed infectious disease cases, each with ≥72 h of continuous operation, were included in the assessment. Colony-forming units (CFU) in air intake, air outlet, and internal tubing, were determined at the following time point, before disinfection, 1 h after disinfection, and 4 h after disinfection. The eligibility criteria were CFU≤20 per sample with no detectable pathogenic bacteria. All data were subjected to statistical analysis. The qualified rate was 0% before disinfection by H2O2, while it was 100% after disinfection for 1 h and 4 h, and CFU in each detection site was significantly lower after disinfection (p＜0.05), and no pathogenic bacterial growth was detected. The tandem H₂O₂ dry mist-disinfecting device is reliable and effective, and the expected effect could be achieved by 1 h disinfection.