1. Department of Dermatology，No. 94826 Hospital of Chinese People’s Liberation Army，Shanghai 200433，China； 2. Department of Dermatology，Changzheng Hospital，Second Military Medical University，Shanghai 200003，China
Abstract：The present report was designed to determine the population structure of genotypes and mating type of Cryptococcus neoformans isolates from Chinese patients infected with human immunodeficiency virus( HIV) living in Shanghai. A total of 12 clinical Cryptococcus neoformans isolates were analyzed by internal transcribed spacer ( ITS) sequence using CLUSTAL W1. 83 and MEGA3. 1 software. The phylogenetic tree was statistically tested via bootstrapping method, and molecular typing was analyzed via polymerase chain reaction ( PCR) -fingerprinting with M13 oligonucleotide as a single primer. Mating type was determined by PCR using two specific primer pairs in accordance with Chaturvedi et al. The results showed that mating-type α, VNⅠ molecular type ( 9 /12, 75% ) is predominant in clinical Cryptococcus neoformans isolates from this group of patients. Our research provides new information on the genetic polymorphisms of Cryptococcus neoformans isolates from Shanghai, China.
