Abstract：The purpose of the current study is to develop a mouse model for exploration of the pathogenesis of dengue virus (DENV) infection. HepG2 cells were transplanted to severe combined immunodeficiency (SCID) mice intraperitoneally to develop a HepG2-SCID mouse chimera model. To confirm the successful transplantation , the concentration of human albumin (hALB) in the serum was determined by sandwich enzyme-linked immunosorbent assay. Following challenge with intraperitoneal injection of DENV2, HepG2- SCID mice were evaluated by detection of virus distribution and histological changes in major organs. HepG2 cells engrafted into SCID mouse could support the replication of DENV2. HepG2-SCID mouse showed viremia and severe organ injuries. The data suggest that DENV2-infected HepG2-SCID chimera model is developed successfully , which would provide a useful tool for studying pathogenesis of DENV infection.
陈艳雷，王娟，高娜，范东瀛，王一松，安静. 登革病毒感染HepG2-严重联合免疫缺陷小鼠模型的建立[J]. 微生物与感染
, 2013, 8(1): 16-20.
CHEN Yan-lei, WANG Juan, GAO Na, FAN Dong-Ying，WANG Yi-Song, AN Jing. Development of HepG2-severe combined immunodeficiency mouse model for dengue virus infection. Journal of Microbes and Infections, 2013, 8(1): 16-20.
