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::: Office ::: Online Submission Manuscript Tracking Peer Review Editor Work Office Work Editor-in-chief   ::: Journal ::: Forthcoming Articles Current Issue Next Issue Archive Email Alert   Read Articles Download Articles   Quick Search       Advanced Search         2023 Vol.18 No.4 Published 2023-08-25 ) [HTML 1KB] [PDF 0KB] ( 5 ) 203 RNA-FISH based on the new generation of hybridization chain reaction to detect enterovirus A71 RNA and its interaction with the viral 3D polymerase RNA-fluorescence in situ hybridization (RNA-FISH) is widely utilized to detect the specific RNA sequences and its contribution in cells or tissues through the complementary hybridization with the fluorescence-labeled nucleotide probes. Given the weak signal, RNA-FISH would be combined with the specific signal amplification technique to improve the signal-to-background ratio. However, due to the low resolution, the traditional signal amplification technology is difficult to eliminate the high background and cannot quantify RNA accurately. It is an obvious obstacle to the application of RNA-FISH. Based on the third generation hybridization chain reaction (HCR v3.0) technology, the half split probes were designed to eliminate the non-specific hybridization background and trigger fluorescence signal amplification. Here, we established the sensitive and specific RNA-FISH based on HCR v3.0 to detect the viral RNA of Enterovirus A71 (EV-A71). Furthermore, RNA-FISH combined with immunofluorescence staining technology (IF) and high-resolution confocal laser imaging, we successfully detected and quantified the viral RNA and 3D polymerase of EV-A71during the viral infection in a single-cell level. We observed that the viral RNA decreased whereas 3D polymerase increased significantly in the late stage of EV-A71 infection. It was apparently different from the quantification by the traditional RT-qPCR and Western Blot, which were based on the total cells during viral infection. It demonstrates that the new generation of RNA-FISH technology based on hybridization chain reaction can overcome the shortcoming of masking the change of virus composition by the increase or decrease of population cell number, so as to truly reflect the change of virus in a single cell. 2023 Vol. 18 (4): 203-210 [Abstract] ( 0 ) [HTML 1KB] [PDF 0KB] ( 8 ) 211 Members of miRNA hsm-let -7 family are expected to be important markers in the differential diagnosis of tuberculous and inflammatory pleural effusions Objective To investigate the differential expression of miRNA in pleural fluid and serum exosomes from patients with tuberculous pleurisy, which is to provide a basis for diagnosis and differential diagnosis of tuberculous pleurisy. Methods 20 patients' serum and pleural fluid who with tuberculous pleurisy (experiment group) and that who have pneumonia with inflammatory pleural effusion (control group 2) , 20 healthy persons' serum (Control Group 1)wer all collected.After isolated exosomes and extrated the total RNA , we analyzed the miRNA expression level with real-time quantitative PCR. Results Compared with control group 1, the level of miRNA expression in serum exosomes from control group2 was increased in different degree, among them the Let-7a-5p, Let-7c-5p, Let-7i-5p and miRNA-126-3p increased significantly, the expression level of miRNA in the serum exosomes from experimental group was higher than that of control group2, especially let-7a-5p, Let-7d-5p, Let-7e-5p, miRNA-126-3p, Let-7g-5p and let-7c-5p (p < 0.05).Compared with control group 2, the expression levels of miRNA in pleural effusion exosomes from the experimental group were higher, and let-7f-5p, Let-7g-5p, let-7d-3p were higher than those in the control group2.The expression of miRNA in pleural effusion and serum exosomes was also significantly different in the experimental group.Conclusion The experession of mirnas in pleural fluid and serum exosomes from patients with tuberculous pleurisy were different from general pneumonia with pleural effusion , the hsm-let-7 family members might be used as a good differential diagnostic index between tuberculous pleural effusion and common inflammatory pleural effusion. 2023 Vol. 18 (4): 211-218 [Abstract] ( 1 ) [HTML 1KB] [PDF 0KB] ( 5 ) 219 Effect of outer membrane vesicles of Salmonella enterica serovar Typhi on the proliferation of colorectal cancer cells The aim of this study was to investigate the effect of outer membrane vesicles of Salmonella enterica serovar Typhi(S. Typhi-OMVs) on the proliferation of colorectal cancer cells under different culture conditions. In this study, ultracentrifugation was used to extract the outer membrane vesicles secreted by S. Typhi under the culture conditions of LB, adding acid, hyperosmotic, oxygen stress-derived LB and LPM, respectively. The morphology, particle size and protein distribution under the electron microscope were used to compare S. Typhi-OMVs cultured in different conditions.The colorectal cancer HT-29, SW480 and CT-26 cells were cultured in vitro. CCK-8 detected the effect of S. Typhi-OMVs (acid, hypertonic, oxygen stress and LPM culture conditions) at different time points on the proliferation of colorectal cancer cells, and the clone formation experiment was further verified. The cell cycle was detected by flow cytometry. To evaluate the drug toxicity of S. Typhi-OMVs in mice through body weight and HE staining sections of liver and kidney tissues.The results showed that there was no significant difference in morphology and size of S. Typhi-OMVs under different culture conditions. Compared with other culture conditions, S. Typhi could secrete more outer membrane vesicles under hyperosmotic stress. Under LB and acid stress, hyperosmotic and oxygen stress and LPM culture conditions, the protein distribution of S. Typhi-OMVs was 37~72KDa, 25~72KDa and 8 ~ 55 kDa respectively. S. Typhi-OMVs(LPM culture condition) can significantly inhibit the proliferation of SW480 and CT-26 cells, and arrest the cell cycle of SW480 cells in G2/M phase, and has no obvious hepatorenal toxicity to mice. It is concluded that S. Typhi-OMVs(LPM culture condition) had direct inhibitory effect on the proliferation of colorectal cancer cells (SW480, CT-26), and it was expected to be a therapeutic drug for colorectal cancer. 2023 Vol. 18 (4): 219-226 [Abstract] ( 4 ) [HTML 1KB] [PDF 0KB] ( 6 ) 227 A case report of a child with sudden enteritis associated with methicillin-resistant Staphylococcus aureus infection and literature review Methicillin-resistant Staphylococcus aureus (MRSA) rarely causes enteritis, and MRSA infection-associated enteritis is generally more common in patients with long-term antibiotic use and after gastrointestinal surgery. In this case, the child had no history of long-term medication and surgery, suddenly developed fever, vomiting, metastatic right lower quadrant pain, suspected appendicitis at the beginning of the disease, and no imaging signs of appendicitis were found after the abdominal CT examination, but the child's intestinal tube was abnormally dilated and pneumofecal retention, and a large amount of dark green watery stool was drained through the canal. After throat swab and fecal etiology test, the child was diagnosed with MRSA infection-related enteritis. After oral anti-infective and symptomatic treatment with vancomycin, the child recovered and was discharged from hospital. This study combined with this case and the literature analysis of MRSA infection-related enteritis to conduct an in-depth analysis of the pathogenesis, clinical manifestations, treatment plan and treatment effect of MRSA infection-related enteritis, so as to provide new ideas for the standardized diagnosis and treatment of MRSA infection-related enteritis. 2023 Vol. 18 (4): 227-234 [Abstract] ( 3 ) [HTML 1KB] [PDF 0KB] ( 7 ) 235 A case of tunnel infection of peritoneal dialysis catheter caused by Actinomyces Turicensis Actinomyces turicensis is a single-cell prokaryotic microorganism, which is the main oral flora of healthy people. If it enters other parts to cause infection, it may cause actinomycosis. This article introduces a case of tunnel infection of peritoneal dialysis catheter caused by Actinomyces turicensis. The patient has been treated with meropenem and cefotaxime, and the disease has improved and discharged. By studying the clinical characteristics, laboratory detection and treatment methods of Actinomyces turicensis infection, this article helps clinicians to become more familiar with the diagnosis and treatment of this kind of disease, and emphasizes that the clinical and laboratory should maintain timely and effective clinical communication in order to achieve the purpose of early diagnosis and treatment. 2023 Vol. 18 (4): 235-239 [Abstract] ( 3 ) [HTML 1KB] [PDF 0KB] ( 6 ) 240 Analysis of 8 cases of bloodstream infection with Bacteroides fragilis in cancer patients Bacteroides Fragilis (BF) is a symbiotic bacterium in human intestinal tract, and also an anaerobic bacterium with relatively high positive rate in clinical work. 12 strains of anaerobic bacteria isolated from 8 cancer patients from June 2020 to December 2021 were identified as Bacteroides Fragilis by MALDI-TOF technique.Analysis of medical records showed that: five patients suffered with colorectal cancer; surgery is the most common predisposing factor for Bacteroides fragilis bloodstream infetion(4/8);Imipenem and cilastatin sodium was seclected for treatment in 4 patients,and the other 4 patients took other different types of antibiotics.All patients were effectively treated with antibiotics, got controll of fever and infection.It turned to be negative for the subsequent blood culture reexamination.This cases analysis of bloodstream infection caused by bacteroides fragilis in cancer patients, is to provide evidence for microbiology detection and clinical diagnosis and treatment . 2023 Vol. 18 (4): 240-245 [Abstract] ( 2 ) [HTML 1KB] [PDF 0KB] ( 8 ) 246 艺兵 罗 A case of acute diarrhea caused by Vibrio furnissii Abstract:Vibrio furnissii is a gram-negative microbacillus isolated from the feces of patients with diarrhea. Vibrio vernisi infection is rarely reported at home and abroad. This paper reports a case of Vibrio Vernisiae infection in a patient with acute diarrhea admitted to the Second People's Hospital of Nanning City. The symptoms of the patient's diarrhea infection were typical, including repeated diarrhea for 4 years, 7-8 times per day. Vibrio Furnissii from this case was indistinctable due to its full biochemical reaction, which was similar to Vibrio fluvialis and different from the identification results by mass spectrometry. Finally, it was confirmed as Vibrio Furnissii by 16S rRNA gene sequencing. According to the growth situation of Vibrio Furnissii, laboratory detection and retrospective analysis of relevant literature reports, it is helpful to improve laboratory personnel's understanding of Vibrio furnissii and clinical diagnosis and treatment of diarrhea caused by rare vibrio. 2023 Vol. 18 (4): 246-250 [Abstract] ( 3 ) [HTML 1KB] [PDF 0KB] ( 3 ) 251 Research progress on the interaction between vaginal microbiome and human papillomavirus infection and cervical lesions The human vaginal microbiota (VMB), which plays a vital role in maintaining health and homeostasis, exhibits low diversity compared to the microbiomes of other organs. Cervical cancer (CC) is a common malignancy in women and has been shown to be highly associated with persistent infection with high-risk human papillomavirus (HPV), and its incidence is second only to breast cancer in the genitourinary system. There is growing evidence that vaginal microbiodysbiosis may play a positive role in the progression of HPV infection and cervical intraepithelial neoplasia (CIN), leading to CC, but there has been little research on the mechanisms driving these associations. This review focuses on the relationship between the vaginal microbiome, persistent HPV infection and cervical dysplasia and the factors that mediate these relationships, which will help to find new targets for VMB-related diseases and further manage HPV-related gynaecological diseases, thereby modifying potential risk factors in clinical practice and guiding clinical treatment of related diseases. 2023 Vol. 18 (4): 251-256 [Abstract] ( 0 ) [HTML 1KB] [PDF 0KB] ( 6 ) Reader Login Author Center Online Submission Author Instruction Layout Art Copyright Agreement News More >>   Other Journal

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