Abstract:Plesiomonas shigelloides belongs to the genus Orthomonas of Enterobacteriaceae. It is a gram-negative bacterium and opportunistic pathogens of human beings. It was firstly found in the gastrointestinal tract, but there was no clear evidence that it was enteropathogenic, and only a few cases supported that it could cause sepsis and gastroenteritis. Vibrio cholerae is a gram-negative bacterium belonging to Vibrio. Serotypes O1 and O139 are the pathogenic bacteria of class A infectious diseases of cholera. They can cause severe vomiting and diarrhea by invasiveness and cholera enterotoxin. Blood flow infection of serotype non-O1/O139 group is occasionally reported in China. This patient was with chronic liver disease. There was no obvious inducement for abdominal pain, diarrhea and vomiting. Blood was collected for culture when they were afraid of cold and shivering. A gram-negative bacilli was detected and identified as Vibrio cholerae by VITEK 2 compact automatic bacterial identification and drug sensitivity analysis system. After qualitative analysis and 16S rDNA gene sequencing, the patient was confirmed to be infected with Plesiomonas shigelloides. According to the results of drug sensitivity test, piperacillin/tazobactam was given for anti infection. After symptomatic treatment of blood transfusion and liver protection, the patient was discharged from hospital with no recurrence. The results of this report suggest that when Vibrio cholerae and other clinically rare bacteria are identified by vitEK-2 Compact, a comprehensive judgment should be made based on bacterial colony morphology, auxiliary biochemical tests and clinical conditions of patients. If necessary, mass spectrometry, gene sequencing and other detection methods can be used for rechecking.
朱卫平,邓洪岩,刘君. VITEK 2 Compact 联合MALDI-TOF MS、16S rDNA基因测序对1株类志贺邻单胞菌的对比鉴定报告[J]. 微生物与感染, 2022, 17(1): 35-40.
ZHU Weiping, DENG Hongyan, LIU Jun. Comparative identification report of a strain of P. shigella by VITEK 2 Compact, MALDI-TOF MS and 16S rDNA gene sequencing . JOURNAL OF MICROBES AND INFECTIONS, 2022, 17(1): 35-40.